- Martina Franca (km 5) famous for its historical city centre and precious XVIII Baroque architecture. There are baroque front doors decorated with apotropaic masks and splendid caryatids. Martina Franca is also famous for its churches founded during the Middle Ages. Every year in July and August the Festival of the Valle D'Itria is held. Martina Franca is also famous for the natural park Bosco of the Pianelle.
- Alberobello (km 9) is unique for its historical city centre as it is the capital of the' Trulli' a peculiar place to live, made in stone. They are part of that area recognized by the UNESCO as The Patrimony of Humanity.
- Fasano (km 10) famous for its Zoo. Fasanolandia is the fun park. There are also the ruins of Egnazia dating back to the II millennium BC. These are cultural site of interest.
- Cisternino (km 10) famous for its little alleys and balconies full of flowers. Cisternino is visited by those who love wine and food, in fact, the town is famous for its meat specialties. There are several butcher shops that in the evening they turn into restaurants by putting tables on the street.
- Polignano a mare (km 25) famous for its position on a cliff overlooking the sea, and rich with grottos and hollows and caves in the rocks.
- Castellana Grotte (km 23) famous for its splendid grottos.
- Ostuni (km 23) called the "white city" because the city walls are white lime. The town is known for its historical centre characterized with baroque architecture.
- Matera (km 75) famous for its rocky churches, caverns and stones. Matera is also recognized by the UNESCO Patrimony of the Humanity.
- Castel Del Monte (km 90) Founded by Frederic II unique for its octagonal shape. It is also the Patrimony of the UNESCO.
- Lecce (km 100) is known for its naturalistic, artistic and archaeological sites. Lecce is also famous for its baroque churches and for the area called Salento where the untouched nature together with the sea gives origin to enchanted landscapes. It is possible from here to visit Otranto and Gallipoli and Capo Santa Maria di Leuca.
- Oasi naturale of Torre Guaceto (km 40)
